Evaluation of third generation anti-HCV enzyme immunoassays.
Artigo
em Inglês
| IMSEAR
| ID: sea-124259
ABSTRACT
The Hepatitis C Virus (HCV) is a major cause of post transfusion hepatitis. The introduction of HCV antibody screening has reduced the risk of post transfusion hepatitis significantly. However, the test is yet to be used routinely in blood banks of several developing countries with limited resources. We have developed an Enzyme immunoassay using synthetic peptides. The test was compared to seven commercial tests available in the Indian market. The test was evaluated using a panel of 90 sera which were chosen from an earlier panel based on detection of HCV RNA by Reverse Transcription Polymerase Chain Reaction RT-PCR. In case of any discrepancy the sera were further analysed by Line immunoassay (LIA). The sensitivity of the in house EIA was 90%. The specificity of the commercial EIAs varied.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Transfusão de Sangue
/
Humanos
/
Técnicas Imunoenzimáticas
/
Sensibilidade e Especificidade
/
Hepatite C
/
Hepacivirus
/
Anticorpos Anti-Hepatite C
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Ano de publicação:
1998
Tipo de documento:
Artigo
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