Experimental infection of laboratory animals with two "FIXED" rabies virus strains by different routes

ABSTRACT

Experimental infection of laboratory animals with two "fixed" rabies virus strains by different routes. By feeding guimea pig brains infected with the Standard Challenge Virus (CVS) rabies strain, 2 albino rats and 1 white mouse became Infected. Transmission was not effected by feeding CVS infected guinea pig tissues to either rats or mice. Feeding of CVS infected mouse brains did not produce infection in rats and mice. The mean mouse LD50 dose by intramuscular injection was 10 2.25 while the corresponding mean mouse l/C LD50 ,dose qf theCVS mouse brain suspensions used was 10 8.07 . Transmission of the Pasteur Variant (PV) rabies virus by gastric intubation of concentrated infective sheep brain suspensions in rats and mice was successful. In addition, mice could also be infected by exposure to aerosols of the same infective materials. the mean mouse intramuscular LD 50 dose of the brain suspensions was 18.5per cent. The mean mouse intracerebral LD5o of the infected materials used was 10-5.1. Virus was not transmitted to untreated white mice kept in contact with infected ones. Confirmation of rabies infection was done by either the WHO Mouse inoculation test and or the fluorescent Antibody Test (F.A.T).