Some aspects of polymorphonuclear leucocyte metabolism

ABSTRACT

The variation in glycolytic lactate production by resting polymorphonuclear leucocytes of guinea pig has been investigated under varied conditions. An important finding in this work indicated that the number of times an exudate is induced in animals contribute to an appreciable change in the rate of aerobic glycolysis. Also, the age of the animals and their intracellular ascorbic acid status showed a significant effect on rate of lactate production by these cells. The nature of anticoagulant used in cell suspension preparation has been found to have a pronounced effect on lactate production. The rate of aerobic glycolysis by resting polymorphonuclear cells and the possible sources of H ions in glycolytic glucose metabolism are discussed.