Detection of Entamoeba histolytica antigen in stool samples by enzyme-linked immunosorbent assay using monoclonal antibodies
Artigo
| IMSEAR
| ID: sea-126878
ABSTRACT
An enzyme-linked immunosorbent assay (ELISA), using monoclonal antibodies, was applied for the detection of Entamoeba histolytica antigen in stool samples obtained from 148 patients with gastrointestinal symptoms, and the results are compared with microscopic findings. Ninety nine positives by microscopy generally had high ELISA OD values. Ninety one stool samples of asymptomatic cyst passers were also investigated by ELISA, and most were found to be positive. Although false positives were observed in both symptomatic and asymptomatic cases, the ELISA appears to be useful for the detection of amoebic antigen(s). However, our results suggest that both immunological methods and microscopic examination are needed for an accurate diagnosis of intestinal amoebiasis.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Mianmar
/
Diarreia
/
Disenteria
/
Entamoeba histolytica
/
Anticorpos Monoclonais
Tipo de estudo:
Estudo diagnóstico
País/Região como assunto:
Ásia
Ano de publicação:
1993
Tipo de documento:
Artigo
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