Preparation of enterotoxigenic Escherichia Coli (heat labile) antisera and its use in BIKEN assay (Part 2)

ABSTRACT

The purified Escherichia Coli (LT) toxin was immunized in rabbits. The specificity of the antisera was demonstrated by Ouchterlony double-gel diffusion tests and by neutralization of the activities of CT and LT to cause morphological changes in Chinese Hamster Ovary cells assays. The optimum titre of the antisera was checked and BIKEN assay was used to check the sensitivity and specificity of the antisera. A total of 965 isolates were used to checked for the identification of LT by BIKEN assay and found that 27 isolates produced LT.