ANTIPROLIFERATIVE EFFECT ON COLON CANCER CELL LINES BY AQUEOUS EXTRACT FROM THE BARK OF MILLINGTONIA HORTENSIS

ABSTRACT

Millingtonia hortensis is a traditional medicinal plant widely used in South-East Asia. This study focused on the antiproliferative effect when comparing between aqueous extract and ethanol extract from the bark of M. hortensis using human colon cancer cell lines, DLD-1, HCT15, SW48 and SW480. In in vitro experiments, the MTT method was used to determine the antiproliferative effect of M. hortensis extract on colon cancer cells. An aqueous extract of M. hortensis significantly reduced the proliferation rate of colon cancer cell lines in a dose-dependent manner. However, no antiproliferative effect was observed by ethanol extract of M. hortensis. This finding indicated that only aqueous extract from the bark of M. hortensis is able to inhibit colon cancer cell proliferation, and its mechanism is now under investigation.