Molecular cloning and expression analysis of evolutionarily conserved stathmin from Gekko japonicus spinal cord.
Indian J Biochem Biophys
;
2009 Aug; 46(4): 289-293
Artigo
em Inglês
| IMSEAR
| ID: sea-135207
ABSTRACT
The cDNA encoding stathmin is identified from the brain and spinal cord cDNA library of Gekko japonicus. It contains a 450 bp open-reading-frame, corresponding to a deduced protein of 149 amino acids. At amino acid level, gecko stathmin shares more than 76.4% identities with vertebrate stathmins, and especially, it shares 100% identity with human stathmin, suggesting that the selective pressure must have been extremely high for the conservation of stathmin during the vertebrates including reptile evolution. Reverse transcriptase polymerase chain reaction (RT-PCR) shows that gecko stathmin is ubiquitously expressed in all tissues examined. In situ hybridization reveals that stathmin transcript mainly appear in the gray matter of spinal cord. The change of stathmin expression in spinal cord after tail amputation is examined by semi-quantitative RT-PCR. Stathmin expression increases at 1 day and 3 day after amputation and decreases to the control level at 1 week. However, the expression level increases again at 2 weeks. These suggest that stathmin may be associated with the immune protection of the injury, as well as in the regeneration of spinal cord.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Medula Espinal
/
Encéfalo
/
Humanos
/
Dados de Sequência Molecular
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Sequência de Bases
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Regulação da Expressão Gênica
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Fases de Leitura Aberta
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Sequência de Aminoácidos
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Clonagem Molecular
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Homologia de Sequência de Aminoácidos
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
2009
Tipo de documento:
Artigo
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