Characterization of erythrosine B binding to bovine serum albumin and bilirubin displacement.
Indian J Biochem Biophys
;
2009 Aug; 46(4): 325-331
Artigo
em Inglês
| IMSEAR
| ID: sea-135212
ABSTRACT
The interaction of erythrosine B (ErB), a commonly used dye for coloring foods and drinks, with bovine serum albumin (BSA) was investigated both in the absence and presence of bilirubin (BR) using absorption and absorption difference spectroscopy. ErB binding to BSA was reflected from a significant red shift of 11 nm in the absorption maximum of ErB (527 nm) with the change in absorbance at λmax. Analysis of absorption difference spectroscopic titration results of BSA with increasing concentrations of ErB using Benesi-Hildebrand equation gave the association constant, K as 6.9 104 M-1. BR displacing action of ErB was revealed by a significant blue shift in the absorption maximum, accompanied by a decrease in absorbance difference at λmax in the difference spectrum of BR-BSA complex upon addition of increasing concentrations of ErB. This was further substantiated by fluorescence spectroscopy, as addition of increasing concentrations of ErB to BR-BSA complex caused a significant decrease in fluorescence at 510 nm. The results suggest that ErB binds to a site in the vicinity of BR binding site on BSA. Therefore, intake of ErB may increase the risk of hyperbilirubinemia in the healthy subjects.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ligação Proteica
/
Espectrometria de Fluorescência
/
Temperatura
/
Bilirrubina
/
Sítios de Ligação
/
Albumina Sérica
/
Soroalbumina Bovina
/
Bovinos
/
Cinética
/
Eritrosina
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
2009
Tipo de documento:
Artigo
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