Involvement of neuronal NO synthase in collateral artery growth.
Indian J Biochem Biophys
;
2011 Aug; 48(4): 270-274
Artigo
em Inglês
| IMSEAR
| ID: sea-135328
ABSTRACT
To evaluate the role of neuronal nitric oxides synthase (nNOS) in collateral artery growth (arteriogenesis), we analyzed the expression pattern of nNOS at distinct time points on RNA and protein levels in a rabbit and a murine model of peripheral arteriogenesis. In the rabbit model, Northern blot analyses revealed a significant upregulation of nNOS at 6 h (1.6-fold), 12 h (2.2-fold) and 24 h (2.0-fold) after induction of arteriogenesis via femoral artery ligation, when compared to the sham operated side. In mice, an upregulation of nNOS was also detected using Northern blot (at 6 h, 12 h) and qRT-PCR (12 h 2.4-fold). On the protein level, nNOS was found to be upregulated 24 h after femoral artery ligation. Immunohistochemical staining showed that nNOS was localized in endothelial and smooth muscle cells of collateral arteries, as well as in skeletal muscle and nerves. In summary, our data provide evidence that nNOS is not constitutively expressed, but is induced during arteriogenesis, playing a role in supplying reactive oxygen species such as H2O2 and low levels of NO.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Artérias
/
Coelhos
/
Imuno-Histoquímica
/
Sequência de Bases
/
Western Blotting
/
Circulação Colateral
/
Primers do DNA
/
Óxido Nítrico Sintase Tipo I
/
Reação em Cadeia da Polimerase em Tempo Real
/
Animais
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
2011
Tipo de documento:
Artigo
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