Assessment of HER-2/neu status in breast cancer using fluorescence in situ hybridization & immunohistochemistry: Experience of a tertiary cancer referral centre in India.
Artigo
em Inglês
| IMSEAR
| ID: sea-135582
ABSTRACT
Background & objective:
Determination of HER2 status in breast cancer has become important to identify potential candidates for anti-HER2 therapy. In this study we compared fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) for the determination of HER2 status in breast cancer patients referred to a tertiary care referral centre.Methods:
A total of 200 cases of invasive breast cancer were evaluated for HER2 status using IHC and FISH and results were compared.Results:
The IHC 3+ (93.9%) and IHC negative (85.9%) cases showed good concordance with the corresponding FISH results; while 66.6 per cent of IHC 2+ cases showed gene amplification by FISH. In addition, hormone receptor expression and HER2 gene status showed a statistically significant inverse association (P<0.05). Interpretation &conclusion:
These findings reaffirm IHC as a prudent first-step to screen tissue samples for HER2 status and to determine suitability for technically demanding FISH test and the dual coloured FISH as a gold standard for determination of HER2/neu status in IHC equivocal cases of breast carcinoma.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Neoplasias da Mama
/
Idoso
/
Feminino
/
Humanos
/
Masculino
/
Biomarcadores
/
Técnicas Imunoenzimáticas
/
Sensibilidade e Especificidade
/
Hibridização in Situ Fluorescente
/
Receptor ErbB-2
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
País/Região como assunto:
Ásia
Idioma:
Inglês
Ano de publicação:
2010
Tipo de documento:
Artigo
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