Mycobacterial Interspersed Repetitive Unit typing in Mycobacterium tuberculosis isolates from Sichuan Province in China.
Artigo
em Inglês
| IMSEAR
| ID: sea-135769
ABSTRACT
Background & objectives:
Emergence and spread of drug resistant Mycobacterium tuberculosis is a serious threat to tuberculosis (TB) control programme. Therefore, the objective of this study was to genotype drug-resistant M. tuberculosis strains isolated from patients in Sichuan, China, using Mycobacterial Interspersed Repetitive Units (MIRU) for epidemiological analysis.Methods:
Drug-resistance testing of M. tuberculosis isolates from pulmonary TB patients was confirmed by proportion method. Twelve MIRU loci were analyzed on 80 drug-resistant and 9 susceptible isolates by polymerase chain reaction and agarose gel electrophoresis. Hunter-Gaston discriminatory index (HGI) values were determined for each 12 MIRU loci for the evaluation of their discrimination power.Results:
Among 12 MIRU loci examined, polymorphic bands could be generated on 11 loci. Sixty five isolates had distinct MIRU patterns, while other 24 belonged to 8 clusters and resistant to at least one anti-TB drug tested. The association between the MIRU patterns and the mutation patterns of drug-resistance relevant target genes was not significant among the drug-resistant isolates. Interpretation &conclusions:
The results showed that with a satisfactory discrimination power exhibited, the 12 loci based MIRU typing could be a valuable tool for epidemiological studies in M. tuberculosis isolates from Sichuan.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Humanos
/
China
/
Reação em Cadeia da Polimerase
/
Técnicas de Tipagem Bacteriana
/
Tuberculose Resistente a Múltiplos Medicamentos
/
Sequências Repetitivas Dispersas
/
Eletroforese em Gel de Ágar
/
Loci Gênicos
/
Frequência do Gene
/
Mycobacterium tuberculosis
País/Região como assunto:
Ásia
Idioma:
Inglês
Ano de publicação:
2011
Tipo de documento:
Artigo
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