Functional estimation of mannose binding lectin associated serine protease (MBL-MASPs) in human serum.
Artigo
em Inglês
| IMSEAR
| ID: sea-135916
ABSTRACT
Background & objectives:
Mannose binding lectin (MBL), a C-type or Ca2+ dependent lectin, plays a major role in lectin pathway of complement activation. MBL deficiency/insufficiency is associated with susceptibility to many infections. It is important to know the association of functional lectin levels with disease condition. Therefore, we carried out this study to develop a simple assay to estimate the functional MBL-associated serine proteases (MBL-MASPs) levels in human serum samples.Methods:
A novel method was developed based on direct haemolysis of mannan coated human erythrocytes in autologous human serum for functional estimation of MBL and associated serine proteases (MBLMASPs complex). Functional MBL-MASPs serum levels in 75 healthy individuals was estimated. Results were compared with those obtained by ELISA based assay.Results:
Lysis of mannan coated human RBC in autologous serum was highly specific and mediated by MBL-MASPs lectin complement pathway. Concentration of MBL-MASPs in serum of normal healthy individuals (n=75) was found to be 1.579 μg/ml (median= 1.149 μg/ml) by the haemolytic assay which was comparable to the values obtained by ELISA method. Interpretation &conclusions:
Our findings showed that the method developed for the estimation of functional MBL-MASPs levels in human serum is simple, cost-effective and comparable with existing ELISA method.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Humanos
/
Ensaio de Imunoadsorção Enzimática
/
Análise de Regressão
/
Lectina de Ligação a Manose
/
Eritrócitos
/
Serina Proteases Associadas a Proteína de Ligação a Manose
/
Hemólise
/
Mananas
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Ano de publicação:
2009
Tipo de documento:
Artigo
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