Your browser doesn't support javascript.
loading
Rapid determination of nevirapine in human plasma by high- performance liquid chromatography.
Artigo em Inglês | IMSEAR | ID: sea-136749
ABSTRACT

Objective:

 To develop and validate a high-performance liquid chromatography (HPLC) method for the determination of nevirapine in human plasma.

Methods:

 A plasma sample and an internal standard were extracted with tert.-butyl methyl ether and determined nevirapine concentration by HPLC method. The limit of quantitation (LOQ), accuracy, precision, specificity, stability and recovery were tested for method validation.

Results:

 Standard curve was linear in the range 0.1 ตg/mL to 20 ตg/mL. The limit of quantitation was 0.1 ตg/ml. Coefficients of variation (CV) of intraday and inter-day precision were less than 4 %. Accuracy was range from 97-101%. The extraction recovery was range from 94-112 %.

Conclusion:

 A rapid, sensitive and specific HPLC method was developed and can be used for determination of plasma nevirapine concentration in adult and pediatric patients infected with HIV.

Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Idioma: Inglês Ano de publicação: 2007 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Idioma: Inglês Ano de publicação: 2007 Tipo de documento: Artigo