Effects of hydroquinone on retinal and vascular cells in vitro.
Indian J Ophthalmol
;
2012 May; 60(3): 189-193
Artigo
em Inglês
| IMSEAR
| ID: sea-139468
ABSTRACT
Aim:
To explore the molecular pathophysiology that might explain the epidemiologic association between cigarette smoke and age-related macular degeneration (AMD) by examining the effects of hydroquinone (HQ), a toxic compound present in high concentration in cigarette smoke-related tar, on human retinal pigment epithelial cells (ARPE-19), rat retinal neurosensory cells (R-28), and human microvascular endothelial cells (HMVEC). Materials andMethods:
ARPE-19, R-28, and HMVEC were treated for 24 h with four different concentrations of HQ (500 μM, 200 μM, 100 μM, 50 μM). Cell viability, caspase-3/7 activation, DNA laddering patterns, and lactate dehydrogenase (LDH) levels were analyzed.Results:
At 50 μM HQ, R-28 cells showed a significant decrease in cell viability compared with the dimethyl sulfoxide (DMSO)-treated controls. At the 100–500 μM concentrations, all three cell lines showed significant cell death (P < 0.001). In the ARPE-19, R-28, and HMVEC cultures, the caspase-3/7 activities were not increased at any of the HQ concentration.Conclusion:
Our findings suggest that the mechanism of cell death in all three cell lines was through non-apoptotic pathway. In addition, neuroretinal R-28 cells were more sensitive to HQ than the ARPE-19 and HMVEC cultures.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ratos
/
Humanos
/
Endotélio Vascular
/
Sobrevivência Celular
/
Células Cultivadas
/
Apoptose
/
Modelos Animais de Doenças
/
Caspase 3
/
Caspase 7
/
Fragmentação do DNA
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Indian J Ophthalmol
Ano de publicação:
2012
Tipo de documento:
Artigo
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