SAD phasing with in-house Cu Kα radiation using barium as anomalous scatterer.
Indian J Biochem Biophys
;
2011 Dec; 48(6): 399-405
Artigo
em Inglês
| IMSEAR
| ID: sea-140207
ABSTRACT
Phasing of lysozyme crystals using co-crystallized barium ions was performed using single-wavelength anomalous diffraction (SAD) method using Cu Kα radiation with in-house source of data collection. As the ion binding sites vary with respect to the pH of the buffer during crystallization, the highly isomorphic forms of lysozyme crystals grown at acidic and alkaline pH were used for the study. Intrinsic sulphur anomalous signal was also utilized with anomalous signal from lower occupancy ions for phasing. The study showed that to solve the structure by SAD technique, 2.8-fold data redundancy was sufficient when barium was used as an anomalous marker in the in-house copper X-ray radiation source for data collection. Therefore, co-crystallization of proteins with barium containing salt can be a powerful tool for structure determination using lab source.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Bário
/
Muramidase
/
Cobre
/
Partículas alfa
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
2011
Tipo de documento:
Artigo
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