A novel tissue microarray instrumentation:The HT-1 tissue microarrayer.
Indian J Pathol Microbiol
;
2012 Jul-Sept 55(3): 314-318
Artigo
em Inglês
| IMSEAR
| ID: sea-142259
ABSTRACT
Background:
Tissue microarray (TMA) is a novel and useful tool to efficiently analyze gene expression in histological tissues.Aim:
Cost-efficient and easy to use automated tissue arrayers will provide a better instrumentation to generate TMAs. Thus, we designed and produced our tissue microarrayer to meet these needs. Materials andMethods:
The HT-1 tissue microarrayer we designed and manufactured consists primarily of four parts, including an instrument to make array pores for the recipient paraffin blocks, a punch needle, an instrument for negative-pressure embedding, and a special manipulator. By using the HT-1, 14 different TMAs were made to accommodate 312 cases of tissues and TMA sections were tested by hematoxylin-eosin (H&E) staining, in situ hybridization, and immunohistochemistry.Results:
Expand Hematoxylin and eosin staining showed that the tissue cylinders were similar, even, and in order on the slides. Most importantly, the HT-1 microarrayer can make array pores in the recipient paraffin block with a single application in seconds. The HT-1 also contains a unique negative pressure system for embedding TMA blocks. In addition, HT-1 can make tissue cylinders with the same levels and depth for equally embedded and sectioning.Conclusions:
The HT-1 tissue microarrayer is a device that is simple, economical and easy to use.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Automação
/
Humanos
/
Análise Serial de Tecidos
/
Patologia Molecular
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Indian J Pathol Microbiol
Ano de publicação:
2012
Tipo de documento:
Artigo
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