Detection of Helicobactor pylori by polymerase chain reaction: A comparison in sample preparation.
Indian J Hum Genet
;
2005 Jan; 11(1): 35-38
Artigo
em Inglês
| IMSEAR
| ID: sea-143326
ABSTRACT
Gastric biopsy samples obtained from 14 patients with upper abdominal pain, clinically diagnosed as acid peptic disease, were analysed for the presence of Helicobacter pylori (H. pylori) by Polymerase Chain Reaction (PCR) using partially (template A) and completely purified DNA (template B). Antigen specific primer was used to analyse the sample by PCR method. The presence of H. pylori in the samples was confirmed by running a positive control. The presence of H. pylori was also detected by urease method using standard protocol. Among the 14 samples studied, 8 showed the presence of H. pylori with both templates A and B. Among these 8 samples only 3 showed positive for the presence of H. pylori with urease method. The present work discusses the results obtained in the detection of H. pylori in template A and B by PCR method.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Tipo de estudo:
Estudo diagnóstico
/
Guia de Prática Clínica
Idioma:
Inglês
Revista:
Indian J Hum Genet
Ano de publicação:
2005
Tipo de documento:
Artigo
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