Telomere instability caused by subtelomeric Y′ amplification and rearrangements in Saccharomyces cerevisiae (ku70 tel1 and ku70 rad50) double mutants.

ABSTRACT

Telomeres solve the end-replication problem. Previous results suggested a relation between Yku70/80 and proteins Tel1 and Rad50 in telomere stabilization. Inactivation of any of these genes lead to a shortening of telomeres, while in ku70 tel1 or ku70 rad50 double mutants a drastic amplification of Y' elements was found. The biological significance of this observation is not clear. To further characterize Y′ amplification 25 strains and isolates of S. cerevisiae were analyzed. As expected, amplification was seen in yku70 tel1 and yku70 rad50 double mutants, but not in other strains. The extent of Y′ amplification was also tested to determine if excessive numbers of Y′ repeats appear. A variation in chromosome lengths within the population of cells has been found. Hybridisation study indicated that chromosomes only increase in length in these double mutants, but never get shorter. A high degree of variability was observed in single cell clones, in spite of their close relationship, indicating that alterations in subtelomeric regions are not stable but occur continuously in these mutants. Therefore, these genes are essential to chromosome stability.