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Analysis of loss of heterozygsity effect on thyroid tumor with oxyphilia cell locus in familial non medullary thyroid carcinoma in Iranian families.
Indian J Hum Genet ; 2012 Sept; 18(3): 340-343
Artigo em Inglês | IMSEAR | ID: sea-145857
ABSTRACT
Material and

Methods:

22 nuclear families (78 persons including 12 patients) with papillary and follicular tumors were selected in a period of six months from Milad hospital. Five microsatellite markers (D19S413, D19S391, D19S916, D19S568, D19S865) on 19p13.2 were selected for genetic analysis. Genomic DNAs was extracted; PCR and polyacrylamide gel electrophoresis method were used for variation detection.

Results:

The results show that 5.4% of the follicular carcinomas and 17.9% of the papillary carcinomas presented LOH at recognition sites. LOH of Papillary carcinoma detected about 13.9% and follicular carcinoma 7.2% in this study. The frequency of informative cases was not similar for each marker D19S413 (41.1%)[1], D19S391 (12.5%), D19S916 (10.7%), D19S568 (1.8%) and D19S865 (3.6%). Loss of hetrozygosity in D19S413 predicts the relation between variation in this region and the disease.

Discussion:

Our findings showed an average of 13.9% LOH in FNMTC cases. Among the five major microsatellites, D19S413 was the most informative for LOH analysis of FNMTC.

Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Tipo de estudo: Estudo prognóstico Idioma: Inglês Revista: Indian J Hum Genet Ano de publicação: 2012 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: IMSEAR (Sudeste Asiático) Tipo de estudo: Estudo prognóstico Idioma: Inglês Revista: Indian J Hum Genet Ano de publicação: 2012 Tipo de documento: Artigo