mRNA and DNA PCR tests in cutaneous tuberculosis.
Indian J Dermatol Venereol Leprol
;
2013 Jan-Feb; 79(1): 65-69
Artigo
em Inglês
| IMSEAR
| ID: sea-147395
ABSTRACT
Background:
The microbiologic diagnosis of cutaneous tuberculosis is difficult because most lesions harbor only a small number of mycobacteria that cannot usually be detected by staining for the organism or by culture. Nucleic acid amplification tests based on the polymerase chain reaction (PCR) are potentially useful in this situation.Aims:
To evaluate the utility of mRNA PCR and DNA PCR in the diagnosis of cutaneous tuberculosis.Methods:
Biopsies from 28 cases of cutaneous tuberculosis and 19 controls with other diseases were subjected to microbiologic tests including direct smears for mycobacteria, culture and both mRNA PCR and DNA PCR. The laboratory was blinded to the clinical diagnosis.Results:
None of the patients or controls showed a positive reaction on mRNA PCR test. Seven of 28 cases and 5 out of 19 controls showed a positive result on DNA PCR test yielding a sensitivity of 25% and a specificity of 73.7%.Conclusion:
The results of PCR tests in cutaneous tuberculosis should be interpreted in the light of clinical and histopathological findings.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Tuberculose Cutânea
/
Biópsia
/
Idoso
/
Feminino
/
Humanos
/
Masculino
/
DNA Bacteriano
/
RNA Mensageiro
/
Estudos de Casos e Controles
/
Criança
Tipo de estudo:
Estudo observacional
Idioma:
Inglês
Revista:
Indian J Dermatol Venereol Leprol
Ano de publicação:
2013
Tipo de documento:
Artigo
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