Improvised microinjection technique for mosquito vectors.
Artigo
em Inglês
| IMSEAR
| ID: sea-147660
ABSTRACT
Background & objectives:
Bio-manipulation technique is of primary importance during the development of transgenic mosquitoes. The study describes the variable factors that influence the viability of medically important mosquito vectors during microinjection.Methods:
Three mosquito vectors belonging to the genus Aedes, Anopheles and Culex were microinjected at different developmental stages of their life cycle viz., egg, larvae, pupae and adult.Results:
The improvisations revealed an increased survivability of biomanipulated mosquitoes during the embryonic and adult microinjection. The study of injecting larvae and pupae resulted in poor survivability. Interpretation &conclusions:
The microinjection protocol was successfully tested on three important mosquito vectors. The critical period after biomanipulation which contributes heavily for the survivability factor was evaluated. The results provide a common protocol for biomanipulation of three mosquito vectors with enhanced survivability.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Idioma:
Inglês
Ano de publicação:
2012
Tipo de documento:
Artigo
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