Mollusc C-reactive protein crosses species barrier and reverses hepatotoxicity of lead in rodent models.
Indian J Exp Biol
;
2013 Aug; 51(8): 623-634
Artigo
em Inglês
| IMSEAR
| ID: sea-149365
ABSTRACT
Achatina fulica C-reactive protein (ACRP) reversed the toxic effects of lead nitrate both in vivo in mice and in vitro in rat hepatocytes restoring the basal level of cell viability, lipid peroxidation, reduced glutathione and superoxides. Cytotoxicity was also significantly ameliorated in rat hepatocytes by in vitro pre-treatments with individual subunits (60, 62, 90 and 110 kDa) of ACRP. Annexin V-Cy3/CFDA dual staining showed significant reduction in the number of apoptotic hepatocytes pre-treated with ACRP. ACRP induced restoration of mitochondrial membrane potential was remarkable. ACRP pre-treatment prevented Pb-induced apoptosis mediated by caspase activation. The antagonistic effect of ACRP may be due to scavenging of reactive oxygen species which maintained the homeostasis of cellular redox potential as well as reduced glutathione status. The results suggest that ACRP crosses the species barrier and it may be utilized as a viable exogenous agent of cytoprotection against heavy metal related toxicity.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ratos
/
Masculino
/
Proteína C-Reativa
/
Mitocôndrias Hepáticas
/
Substâncias Perigosas
/
Peroxidação de Lipídeos
/
Sobrevivência Celular
/
Western Blotting
/
Espécies Reativas de Oxigênio
/
Ratos Sprague-Dawley
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2013
Tipo de documento:
Artigo
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