Analysis of methionine synthase reductase polymorphism (A66G) in Indian Muslim population.
Indian J Hum Genet
;
2013 Apr; 19(2): 183-187
Artigo
em Inglês
| IMSEAR
| ID: sea-149427
ABSTRACT
BACKGROUND AND OBJECTIVES:
Methionine synthase reductase (MTRR) is a vital enzyme of homocysteine/methionine metabolic pathway and is required for the conversion of inactive form of methionine synthase (MTR) to its active form. A clinically important allelic variant of MTRR A66G, with less enzymatic activity is reported with worldwide prevalence rate of ~ 30%. The present study was designed to determine the frequency of MTRR A66G polymorphism in rural Sunni Muslim population of Eastern Uttar Pradesh. MATERIALS ANDMETHODS:
Total 56 subjects were analyzed for MTRR A66G polymorphism. A66G mutation analysis was carried out according to the polymerase chain reaction-restriction fragment length polymorphism method of Wilson et al. [1] amplification with MTRR specific primers followed by amplicon digestion with NdeI enzyme was used for the identification of different MTRR genotypes in subjects. RESULTS ANDDISCUSSION:
The AA genotype was found in 5 subjects, AG in 23 subjects, and GG genotype in 28 subjects. Genotype frequencies of AA, AG, and GG were 0.089, 0.41, and 0.5 respectively. The allele frequency of A allele was found to be 0.298 and G allele was 0.705.CONCLUSION:
It is evident from the present study that the percentage of homozygous genotype GG and frequency of G allele is high in the target Muslim population.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Polimorfismo Genético
/
Idoso
/
Humanos
/
Adolescente
/
Adulto
/
Predisposição Genética para Doença
/
Polimorfismo de Nucleotídeo Único
/
Códigos de Ética
/
Grupos Populacionais
/
Alelos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Indian J Hum Genet
Ano de publicação:
2013
Tipo de documento:
Artigo
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