Quantification of Colchicine in Seed and Tuber samples of Gloriosa superba by High Performance Liquid Chromatography method.

ABSTRACT

To quantify the colchicine (alkaloid) present in Gloriosa superba by High Performance Liquid Chromatography (HPLC) method. Gloriosa superba species are known for their content of colchicine, a valuable alkaloid in the seeds and tubers (corms). HPLC analysis was carried out to confirm the presence of colchicine in Gloriosa superba. Treatment of gibberellic acid and Psedomonas aerugenosa improved the content of colchicine in seeds and tubers. Seeds of Gloriosa superba were rich in colchicines content than the tubers sample.