Evaluation of In-vitro antibacterial and anticandidal activity of Sapium sebiferum L.

The Sapium sebiferum leaf extracts were determined for their antimicrobial activities against Staphylococcus aureus, Micrococcus luteus ,Pseudomonas aeruginosa, Salmonella typhi, Klabsiella oxytoca, Listeria monocytogenes, E.coli, Saccharomyeces cereviceae and Candida albicans. The antimicrobial activities of these strains were compared with standard antibiotics (amoxicillin). Results obtained showed that all the extracts except aqueous (hot and cold) were effective against all the microbial strains. The methanolic extract of Sapium sebiferum leaves was most effective against all the bacterial strains and thus displayed highest zone of 21.0 mm at concentration of 100 mg/ml against Listeria monocytogenes ( MIC value is 25 mg/ml). Salmonella typhi and Klabsiella oxytoca did not show any activity. The anticandidal activity of methanolic, ethanolic and petroleum extracts showed maximum inhibition zone of 24.0 mm (MIC value is 25mg/ml).