Studies on extended beta lactamase producing, biofilm forming clinical bacterial pathogens and its invitro inhibition by Actinobacterial extracts.

ABSTRACT

At present scenario, the extended spectrum beta lactamase (ESBL) producing bacterial pathogen causes various life threatening infections especially by the members of the family Enterobacteriaceae in hospital settings. In order to study the prevalence of ESBLs in Kanchipuram hospital, the bacterial strains were isolated from patients having Urinary tract infections (UTI), diabetic foot ulcer, pregnancy women’s, surgical wound infections, deep wounds, and genitourinary tract problems. Totally 40 bacterial isolates were recovered from 30 samples and the isolates were identified as Escherichia coli (45%), Pseudomonas sp, (25%) and Klebsiella sp (30%). The ESBL production was confirmed with third generation cephalosporins (cetixime, cephoxitin, ceftazidime, cetepime, ceftriaxone, ceftazidime/clavulanic acid) using the Kirby- bauer disc diffusion method and also by double disc diffusion method. The highest ESBL production was found among E. coli (42%), followed by Pseudomonas sp. (25%) and Klebsiella sp (20%). All the ESBL producer were tested for biofilm formation by tube method in which E.coli (43%) was found to be the good biofilm producer followed by the Klebsiella sp (31%) and Pseudomonas sp (25%). An attempt was also made to study the in-vitro inhibition of biofilm forming ESBL pathogens by actinobacterial extracts by disc diffusion method. Of the five actinobacterial extracts tested, extracts produced from the strain MA7 inhibited (8-12 mm zone of inhibition) all the biofilm forming ESBL pathogens. Further purification and characterisation of active compound from actinobacterial strain MA7 is in progress.