Oxidative stress causes plasma protein modification.
Indian J Exp Biol
;
2015 Jan; 53(1): 25-30
Artigo
em Inglês
| IMSEAR
| ID: sea-154987
ABSTRACT
We investigated the effect of oxidative systems on plasma proteins using Chloramine-T, a source of free radicals. Plasma specimens from 10 healthy volunteers were treated with 40 mmol/L Chloramine-T (11 v/v). Total protein and plasma carbonyl levels were evaluated spectrophotometrically. Identification of plasma proteins modifications was performed by SDS-PAGE, protein and lipid electrophoresis. Protein fragmentation was evaluated by HPLC. Total protein levels of oxidised plasmas were significantly lower (4.08 ± 0.12 g/dL) than control (7.86 ± 0.03 g/dL) (P <0.01). Plasma carbonyl levels were higher (1.94 ± 0.38 nmol/mg protein) in oxidised plasma than that of control (0.03 ± 0.01 nmol/mg protein) (P<0.01). Plasma oxidation had no significant effect on the levels of proteins and lipids. Protein fragmentations were detected in oxidised groups compared to those of the control. We conclude that protein modifications have direct effect on the protein functions, which are related to stress agent, its treatment period(s), and the methodology used for evaluating such experimental results.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Humanos
/
Proteínas Sanguíneas
/
Cromatografia Líquida de Alta Pressão
/
Estresse Oxidativo
/
Eletroforese em Gel de Ágar
/
Eletroforese em Gel de Poliacrilamida
Tipo de estudo:
Estudo de etiologia
Idioma:
Inglês
Revista:
Indian J Exp Biol
Ano de publicação:
2015
Tipo de documento:
Artigo
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