Association of Epstein Barr virus deoxyribonucleic acid with lung carcinoma.
Indian J Pathol Microbiol
;
2013 Oct-Dec 56 (4): 359-364
Artigo
em Inglês
| IMSEAR
| ID: sea-155916
ABSTRACT
Context Lung cancer is the leading cause of cancer death worldwide. In addition to smoking, a variety of other contributing factors, including viral infection, have been suggested in tumorigenesis. Epstein Barr virus (EBV), which is linked to various malignancies, seems to be a good candidate. Aims:
The aim of this study was to investigate the association of EBV with lung carcinomas. Settings andDesign:
A total number of 90 formalin fi xed paraffi n embedded lung tissue samples including 48 cases of lung cancers (18 squamous cell carcinomas [SCCs], 18 adenocarcinomas and 12 small cell carcinomas) and 42 non-tumoral samples (control group), were retrieved from the pathology archive. Materials andMethods:
Following deoxyribonucleic acid extraction, polymerase chain reaction (PCR) was performed using an EBV-Eph PCR kit. The positive cases were studied immunohistochemically for the expression of EBV-late membrane protein-1 (EBV-LMP-1) in tumoral tissues. Statistical Analysis Used The t-test and Fisher exact test were used and P < 0.05 was considered statistically signifi cant.Results:
Five of our cases, including four SCCs and one adenocarcinoma and two control samples showed a positive reaction in PCR. All positive tumoral cases showed diffuse staining with LMP-1 in immunohistochemistry.Conclusions:
We found a signifi cant difference in the presence of the EBV genome in cases of lung SCC compared to other lung lesions (P = 0.02). According to our data, EBV is not at major play in the nonlymphoepithelioma- like cancers of the lung in general, but may have a role in the tumorigenesis of some lung SCCs.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Idioma:
Inglês
Revista:
Indian J Pathol Microbiol
Ano de publicação:
2013
Tipo de documento:
Artigo
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