Diagnostic accuracy of morphologic identification of filamentous fungi in paraffin embedded tissue sections: Correlation of histological and culture diagnosis.
Indian J Pathol Microbiol
;
2014 Oct-Dec 57 (4): 583-587
Artigo
em Inglês
| IMSEAR
| ID: sea-156130
ABSTRACT
Aims and Objectives:
The aim was to investigate the correlation between histological and culture diagnosis of fi lamentous fungi. Materials andMethods:
Tissue sections from biopsy samples stained with Hematoxylin and Eosin and special stains from samples of chronic invasive/noninvasive sinusitis and intracranial space occupying lesions during 2005-2011 diagnosed to have infection due to fi lamentous fungi were reviewed. The histopathology and culture diagnoses were analyzed for correlation and discrepancy.Results:
There were 125 samples positive for fi lamentous fungi on biopsy. Of these 76 (60.8%) were submitted for culture and fungi grew in 30 (39.97%) samples. There was a positive correlation between histological and culture diagnosis in 25 (83.33%) samples that included Aspergillus species (16/19), Zygomycetes species (8/10) and dematiaceous fungi (1/1). The negative yield of fungi was more in Zygomycetes species (20/30) when compared to Aspergillus species (25/44). There was a discrepancy in diagnosis in 5/30 (16.67%) samples which included probable dual infection in two, and dematiaceous fungi being interpreted as Aspergillus species in three samples.Conclusion:
Histopathology plays a major role in the diagnosis of infection due to fi lamentous fungi, especially when cultures are not submitted or negative. The discrepancy between histological and culture diagnosis was either due to dematiaceous fungi being interpreted as Aspergillus species or probable dual infection.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Indian J Pathol Microbiol
Ano de publicação:
2014
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS