Use of MALDI-TOF MS technique for rapid identification of bacteria from positive blood cultures.
Indian J Med Microbiol
;
2014 Oct-Dec ; 32 (4): 419-422
Artigo
em Inglês
| IMSEAR
| ID: sea-156959
ABSTRACT
We evaluated the feasibility of same‑day routine aerobic bacterial identification using the following procedures Picking colonies from 4 and 6 h incubated subculture from positive blood culture bottle and analyzing them by matrix‑assisted laser desorption/ionisation time‑of‑flight mass spectrometry (MALDI‑TOF MS). The matched identification rate of this procedure at the species level was 80.6% (141/175) for the 4‑h cultures compared with overnight cultures and 90.9% (159/175) for the 6‑h cultures. Thus, our technique provides an easy and rapid method for identification of aerobic bacteria in routine clinical microbiology laboratories.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Indian J Med Microbiol
Assunto da revista:
Microbiology
Ano de publicação:
2014
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS