Rapid and simple method of photobleaching to reduce background autofluorescence in lung tissue sections.
Indian J Biochem Biophys
;
2015 Feb; 52 (1): 107-110
Artigo
em Inglês
| IMSEAR
| ID: sea-157962
ABSTRACT
Autofluorescence exhibited by tissues often interferes with immunofluorescence. Using imaging and spectral analysis, we observed remarkable reduction of autofluorescence of formalin fixed paraffin embedded tissues irradiated with light prior to incubation with immunofluorescent dyes. The technique of photobleaching offers significant improvement in the quality and specificity of immunofluorescence. This has the potential for better techniques for disease diagnosis.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Espectrometria de Fluorescência
/
Anticorpos Antinucleares
/
Imunofluorescência
/
Fotodegradação
/
Pulmão
Idioma:
Inglês
Revista:
Indian J Biochem Biophys
Ano de publicação:
2015
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS