Development of touch down‑multiplex PCR for the diagnosis of toxoplasmosis.
Indian J Med Microbiol
;
2015 Apr-Jun; 33(2): 271-273
Artigo
em Inglês
| IMSEAR
| ID: sea-159536
ABSTRACT
Purpose:
The diagnosis of toxoplasmosis is challenging since conventional methods like culture and immunofluorescence are not universally available. Serology, which is used regularly might be negative during early phase of infection and in immunosuppressed patients or may remain positive for a long time. Several molecular tests have been used for the diagnosis of toxoplasmosis, but none of them have an internal control which would inform us regarding the presence of polymerase chain reaction (PCR) inhibitors thus, undermining the confidence of a laboratory physician. Materials andMethods:
We designed a multiplex PCR containing primers targeting human beta globin gene which would act as internal control and two primers against the B1 gene and 5s gene which aid in sensitive detection of T. gondii.Results:
Multiplex PCR had a sensitivity of 83.3% and specificity of 100%.Conclusion:
Multiplex PCR may provide a sensitive and specific tool for diagnosis of human toxoplasmosis.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Indian J Med Microbiol
Assunto da revista:
Microbiology
Ano de publicação:
2015
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS