Analysis Of Y-Linked Lineage Markers In Indian Populations.

ABSTRACT

Recently, Y-linked markers have been found extremely useful tools in population genetics and evolutionary biology. We have studied a tetranucleotide marker, DYS19 and a bialleleic marker DYS287, an Alu polymorphic marker (YAP) in five ethnic groups of India. Blood samples were collected from 215 random healthy males and DNA was extracted by using a simple salt precipitation method. DNA samples, after amplification by polymerase chain reaction (PCR), were electrophoresed. The detection of alleles was done by silver staining and reconfirmed by using a fragment analyser programme in an ALF express sequencer (Pharamacia). A total of four alleles were observed at DYS19 locus in all the five ethnic groups. Allele 194bp was found to be the predominant one in all the groups. The absence of YAP was found in all the groups.