Time And Temperature Effects On In-Vitro Sperm Chromatin and Stability.

ABSTRACT

Semen samples were collected both from men with or without children. Sperm wash preparations were made and the samples were studied at different temperatures and times of incubations for genome packaging efficiency. It is found that sperm chromatin decondensation study could be optimised with SDS reagent if the temperature was 37 degrees and time of incubation was 30 minutes. Similarly, SDS & EDTA, optimum time was 15 minutes at body temperature, thus affecting results of the study. Below, the body temperature the reaction is slow and does not mimick the in-vivo process.