Synthesis of L-asparaginase by Serratia marcescens (Nima).

ABSTRACT

The production of L-asparaginase by two mutants of Serratia marcescens grown on 14 different media was studied. The enzyme content increased from trace levels to 2·4 international units per ml when the organisms were grown in glycerol-peptone yeast extract medium. Glucose was the best carbon source under aerobic conditions. The enzyme content increased when L-asparagine was present in the growth medium.