Human erythrocyte specific lectin from the seeds of Indian coral tree, Erythrina variegata Linn, var. orientali Linn, Merrill.
J Biosci
;
1983 Dec; 5(suppl_1): s25-s30
Artigo
em Inglês
| IMSEAR
| ID: sea-160272
ABSTRACT
Human erythrocyte specific lectin was isolated from the seeds of Erythrina variegata Linn. var. orientalis Linn. Merrill. The lectin preferentially agglutinated erythrocytes in the sequence of O>B>A = AB. The lectin was purified 19-fold by affinity chromatography on acid treated sepharose 4B with an yield of 81%. The purified lectin was found homogeneous on polyacrylamide gel electrophoresis. The erythroagglutination reaction was inhibited by N-acetyl-D-galactosamine, D-galactose and lactose at very low concentration. The haemagglutination by the purified lectin was not inhibited by different hexose and pentose sugars even at high concentration. The purified lectin was a glycoprotein and agglutinated leucocytes at 3 μg protein concentration. The lectin induced transformation of peripheral blood lymphocytes in cultures
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Idioma:
Inglês
Revista:
J Biosci
Ano de publicação:
1983
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS