(α-Pyridyl) methyl phosphoro-bis-triazolide as a new phosphorylating reagent for internucleotide bond formation.
J Biosci
;
1988 Jun; 13(2): 189-199
Artigo
em Inglês
| IMSEAR
| ID: sea-160664
ABSTRACT
(α-Pyridyl)methyl phosphoro-bis-triazolide has been found to be a reagent of choice for phosphate protection in oligodeoxyribonucleotide synthesis. The reagent has been used successfully to phosphorylate all the four 5'-and N-protected deoxynucleosides. The resulting 3'-phosphorylated derivatives were found to be fairly stable as either triethyl ammonium salts or cyanoethyl derivatives. The phosphorylated derivatives were used in the preparation of the dimers TpT and d(ApT) in solution phase and a tetramer, TTTT, and a hexamer, d(ATATAT), on solid phase using glass support. The method gave excellent yields. Considerably reduced condensation time (6-9 min) and practically no cleavage of the internucleotide bond during the removal of the group are the advantages.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Idioma:
Inglês
Revista:
J Biosci
Ano de publicação:
1988
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS