Molecular characterization of shiga like toxin-producing Escherichia coli (STEC) isolates from pigs oedema.
Artigo
em Inglês
| IMSEAR
| ID: sea-16140
ABSTRACT
BACKGROUND & OBJECTIVE:
An oedema outbreak occurred in a Guwahati pig farm. Escherichia coli isolates from different necropsy samples collected from the dead piglets with oedema were characterized to confirm the virulence.METHODS:
Haemolytic E. coli isolates recovered from liver, lung and intestine of pigs with oedema were examined for presence of genes encoding pathogroups such as enteropathogenic Escherichia coli (EPEC), (eae/bfpA), enteroaggregative Escherichia coli (EAggEC), (eagg), enterotoxigive Escherichia coil (ETEC), (elt/est) and shiga like toxin producing Escherichia coli (STEC), (stx1/ stx2) by PCR and molecular typing by randomly amplified polymorphic DNA-PCR (RAPD-PCR).RESULTS:
The three haemolytic E. coli recovered from diseased pigs were STEC because of presence of the stx2 and eae genes. Analysis by RAPD-PCR indicated that two of the three isolates were genetically related. INTERPRETATION &CONCLUSION:
The isolation of STEC isolates from pigs with oedema was shown. Although the three isolates were untypable, presence of eae and stx2 genes clearly indicated these as prime cause of pig oedema disease. Further, demonstration of STEC in pigs becomes a public health concern, as pigs are potential reservoir of such agents, which may cause human illness.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
DNA Bacteriano
/
Sequência de Bases
/
Surtos de Doenças
/
Epidemiologia Molecular
/
Toxina Shiga
/
Sus scrofa
/
Edematose Suína
/
Infecções por Escherichia coli
/
Escherichia coli Shiga Toxigênica
/
Índia
País/Região como assunto:
Ásia
Idioma:
Inglês
Ano de publicação:
2008
Tipo de documento:
Artigo
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