Effects of substitutions at position 180 in the Escherichia coli RNA polymerase σ70 subunit.
J Biosci
;
2011 Mar; 36(1): 43-54
Artigo
em Inglês
| IMSEAR
| ID: sea-161510
ABSTRACT
In order to investigate the role of His180 residue, located in the non-conserved region of the σ70 subunit of Escherichia coli RNA polymerase, two mutant variants of the protein with substitutions for either alanine or glutamic acid were constructed and purified using the IMPACT system. The ability of mutant σ70 subunits to interact with core RNA polymerase was investigated using native gel-electrophoresis. The properties of the corresponding reconstituted holoenzymes, as provided by gel shift analysis of their complexes with single- and double-stranded promoter-like DNA and by in vitro transcription experiments, allowed one to deduce that His180 influences several steps of transcription initiation, including core binding, promoter DNA recognition and open complex formation.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Idioma:
Inglês
Revista:
J Biosci
Ano de publicação:
2011
Tipo de documento:
Artigo
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