Excised radicle tips as a source of genomic DNA for PCR-based genotyping and melting curve analysis in cotton.
J Biosci
;
2013 Mar; 38(1): 167-172
Artigo
em Inglês
| IMSEAR
| ID: sea-161803
ABSTRACT
Genomic DNA isolation in cotton is complicated because of the presence of secondary metabolites that are inhibitory to PCR amplification. We report here that radicle tips, but not other parts of cotton seedlings, yield high-quality DNA that is readily amenable for PCR. The radicle-tip-excised seedlings retain viability because of the formation of adventitious roots. We demonstrate the utility of this method in distinguishing homozygotes from heterozygotes in a cotton breeding population and in hybrid seed purity testing.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Idioma:
Inglês
Revista:
J Biosci
Ano de publicação:
2013
Tipo de documento:
Artigo
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