HPTLC Methods for Determination of Gemifloxacin Mesylate in Rabbit Plasma.
Artigo
em Inglês
| IMSEAR
| ID: sea-163508
ABSTRACT
Aim:
A new, simple, rapid, very sensitive and accurate high performance thin-layer chromatographic (HPTLC) method has been developed and validated for estimation of Gemifloxacin in rabbit plasma. StudyDesign:
Validation study.Methodology:
HPTLC was performed on silica gel 60F254 plates with ethanol ethyl acetate hexane, 271 (v/v), as mobile phase. Densitometry scanning was performed in absorbance mode at λ=254 nm.Result:
The RF value was 0.21. The response was a linear function of concentration over the range 0.1–0.7μg mL−1 (r2=0.996). A maximum recovery of drug from plasma was obtained by using chloroform and glacial acetic acid. Mean extraction recovery was 80%. Intra-day and inter-day precision (% RSD) of the assay were in the range 1.19–2.85% and accuracy was 1.7-5.66%Conclusion:
This method can be applied to pharmacokinetic studies in rabbit plasma.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Idioma:
Inglês
Ano de publicação:
2014
Tipo de documento:
Artigo
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