Molecular characterization of Shiga Like Toxin-Producing Escherichia Coli (STEC) isolates from broiler and local variety of poultry.

ABSTRACT

Escherichia coli isolates from different samples of droppings collected from the different farms of broiler and local poultry were characterized to confirm the virulence. E. coli isolates recovered from different farms were examined for presence of genes encoding patho groups such as shiga like toxin producing Escherichia coli (STEC), (stx1/stx2) by Duplex PCR and then analysed the PCR Amplicons by AGE. The three and four isolates of E. coli recovered from local and boiler poultry were STEC because of presence of the stx1 and stx2 genes. Presence of stx1 and stx2 genes clearly indicated these as prime cause of pathogenecity. Further, demonstration of STEC in poultry becomes a public health concern, as poultry are potential reservoir of such agents, which may cause extra intestinal diseases like haemolytic uremic syndrome and thrombocytopenic purpurea by stx1 &2 and these toxins kill vascular endothelial cells.