Analysis of methyl parathion in biological samples using thin layer chromatography.

ABSTRACT

Pesticides are major contaminating chemicals in agriculture environment and a hazard to exposed population. The pesticides form a strong class of environment pollutants, as they are sometimes nonbiodegradable, damaged not only the environment and agriculture but also have entered into the food chain thereby affecting health and development. Methyl parathion is a broad-spectrum organophosphorus insecticide generally used to control a variety of insects. The present study was planned to develop a new method for analysis of Methyl parathion in human blood samples using thin layer chromatography technique, which is simple and quick. Methyl Parathion was extracted from blood using solvent extraction methods and then identified on the TLC plates. For chromatographic separation, various binary and tertiary solvent systems were used and for detection on developed plates, palladium chloride reagent was used which successfully increased the sensitivity without dispensing with the simplicity of the method. Statistical analysis was performed on four solvent systems namely benzene chloroform (8020), hexane carbontetrachloride (5050), hexane carbontetrachloride (2080), hexane propanol (2080) which included the calculation of mean Rf value, value of standard deviation and coefficient of variance. It is evident from the statistical data that hexane carbontetrachloride (5050), hexane carbon tetrachloride (2080) is preferably good solvent systems for parathion identification by thin layer chromatography.