Production Optimization of Extracellular L-Asparaginase Through Solid- State Fermentation By Isolated Bacillus Subtilis.

ABSTRACT

L-asparaginase has been used as anti-tumor agent for the treatment of acute lymphoblastic leukemia and food processing aid to reduce the formation of cancer causing acrylamide. Extracellular Lasparaginase production was optimized through solid state fermentation using ground nut cake by isolated Bacillus subtilis. which was not reported in literature.Optimum production of L-asparaginase enzyme (18.4U/ml) was obtained after 48h of incubation at 370C moisture content of 70% and at pH 7.