In Vitro Regeneration from Shoot Tip and Nodal Explants of Simarouba Glauca DC, a Promising Biodiesel Tree.
Artigo
em Inglês
| IMSEAR
| ID: sea-164121
ABSTRACT
An efficient regeneration protocol was developed from shoot tip and nodal explants of Simarouba glauca DC, a promising biodiesel plant. Nodal explants appeared to have better regeneration capacity than shoot tip explants (40%) in the tested media. The highest regeneration frequency (90%) and shoot number (7.00 ± 1.00 shoots per explants) were obtained in nodal explants in Murashige and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP) 4.43 μM and α-naphthalene acetic acid (NAA) 5.36 μM.Induced shoot buds were multiplied and elongated on the MS medium supplemented with BAP (4.44 μM), NAA (5.36 μM) and TDZ (Thidiazuron) 2.27 μM with 9.66±0.33 (mean length 5.35±0.32 cm) and 9.00±0.57 (mean length 4.51±0.15cm) shoots using nodal segments and shoot tip explants, respectively. Halfstrength woody plant medium (WPM) containing 2.46μM indole-3-butyric acid (IBA) produced the maximum number of roots (6.00±1.15). The rooted plantlets were hardened on MS basal liquid medium and subsequently in polycups containing sterile soil and vermiculite (11) and successfully established in pots.
Simarouba gluca; regeneration; biodiesl tree; acclimatization; Abbreviations:; BAP -6-benzyl amino purine; CaCl2 –Calcium chloride; IAA - Indole-3-acetic acid; IBA - Indole-3-butyric acid; KNO3 –Potassium nitrate; MS- Murashige & Skoog medium; NAA –α-naphthalene acetic acid; NH3NO3–Ammonium nitrate; TDZ –Thidiazuron; WPM- Woody plant medium
Texto completo:
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Índice:
IMSEAR (Sudeste Asiático)
Idioma:
Inglês
Ano de publicação:
2013
Tipo de documento:
Artigo
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