Detection of high molecular weight IgG fibronectin complexes in various types of Acute Myeloid Leukemia – A study in third world country.

ABSTRACT

To detect presence of high molecular weight complexs of lgG and fibronectin, plasma of patients with acute myloid leukemia was examined by polyethylene glycol (PEG) precipitation, analytical ultracentrifugation, and and immunoaffinity chromatography. ultracentrifugation identified abnormal circulating high molecular Weight lgG in all patients. this was precipitated by PEG and was shown by exclusion chromatography to contain IgG in a high molecular weight form. Examination of plasma by immunoaffinity chromatography supported evidence for complex formation between IgG and fibronectin and further showed that abnormal high molecular weight IgG complexes are a prominent feature of Acute Myeloid Leukemia and implicate lgG fibronectin complex formation.