Development of RP-HPLC method for the estimation of Rasagiline mesylate in bulk and tablet dosage forms.

ABSTRACT

A simple RP-HPLC method for the determination of Rasagiline Mesylate in bulk and tablet dosage form was devel-oped. Numerous HPLC conditions were tested for determination of rasagiline. The best result was achieved by using Purosphere star RP-18, (150×4.6mm), 5μm column and a mobile phase consisting of Potassium Orthophosphate Acetonitrile (6040 v/v) adjusted to pH 7.0(±0.05) with Ammonia solution, a flow rate of 1.5 ml/min with ultraviolet detection at 210nm. The correlation coefficients for calibration curves within the detection range of 5-30μg/ml were 0.9993. The within and between-day precision was determined for both retention time and peak area. The retention time of rasagiline is 6.0 minutes.