Rapid technique for the detection of carcinogens using mice liver microsomes.
Artigo
em Inglês
| IMSEAR
| ID: sea-17283
ABSTRACT
Mice liver microsomes were prepared at low g (10,000 g) force by Ca(2+)-aggregation method and were used for the detection of carcinogens by degranulation technique. RNA/protein ratio of these microsomes was 0.177 which was comparable to rat liver microsomes. Per cent degranulation with two carcinogens (O-dianisidine and benzidine) at 20 micrograms concentration was 21 per cent on the basis of RNA/protein ratio basis with both the carcinogens. At 40 micrograms concentration the per cent degranulation was observed to be almost double. Both the carcinogens required NADPH for their activation.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Masculino
/
RNA
/
Microssomos Hepáticos
/
Testes de Carcinogenicidade
/
Proteínas
/
Degranulação Celular
/
Animais
/
Camundongos
/
NADP
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Ano de publicação:
1991
Tipo de documento:
Artigo
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