Preparation & standardization of the goat lung surfactant extract.
Artigo
em Inglês
| IMSEAR
| ID: sea-17303
ABSTRACT
Exogenous goat lung surfactant extract (GLSE) was obtained by chloroform-methanol extraction of the centrifuged lung lavage. Four lungs provided around 250 mg of surfactant. GLSE has a phospholipid content of 50 percent, cholesterol of 1.5 percent and protein content under 1 percent. The preparation was bacteriologically sterile and had a shelf life of at least one month at 4 degrees C. Endotracheal administration of surfactant (50 mg/kg) in a rat lung model rendered surfactant deficient by saline lavage, restored the compliance characteristics to normal, thus documenting biological activity of the preparation. The cost of a course of 2 doses of surfactant replacement therapy was estimated to be as low as Rs 500/-. The study opens up the possibility of surfactant replacement therapy for the management of neonatal respiratory distress syndrome in India by an indigenous product at an affordable cost.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Ratos
/
Padrões de Referência
/
Extratos de Tecidos
/
Surfactantes Pulmonares
/
Cabras
/
Pulmão
/
Animais
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Ano de publicação:
1998
Tipo de documento:
Artigo
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