E test as an alternative to conventional MIC determination for surveillance of drug resistant Streptococcus pneumoniae.
Artigo
em Inglês
| IMSEAR
| ID: sea-17339
ABSTRACT
A commercial E test was compared with the standard agar dilution method for the determination of minimum inhibitory concentration (MIC) of penicillin, erythromycin, chloramphenicol and cefotaxime for 36 strains of Streptococcus pneumoniae from patients with invasive diseases. Additional strains were tested for MIC values for penicillin (6), erythromycin (14) and cefotaxime (13) for a better statistical evaluation. Besides, 5 reference standards with predetermined MIC values obtained from WHO pneumococcal reference center at Copenhagen, Denmark were tested for penicillin and erythromycin, for quality assessment using both agar dilution as well as E test methods. An overall agreement within +/- 2 dilutions was noted for 97 per cent of the strains tested for all the antimicrobials. A high degree of correlation was noted for erythromycin (r = 1), penicillin (r = 0.99), chloramphenicol (r = 0.95) and cefotaxime (r = 0.9). In MIC determination of a single antimicrobial for diagnostic purpose, E test was found to be more cost effective than conventional agar dilution method. E test was simple to perform, easy to interpret and a valid method for MIC determination of antimicrobials for S. pneumoniae in our center.
Texto completo:
DisponíveL
Índice:
IMSEAR (Sudeste Asiático)
Assunto principal:
Streptococcus pneumoniae
/
Humanos
/
Resistência Microbiana a Medicamentos
/
Testes de Sensibilidade Microbiana
Tipo de estudo:
Guia de Prática Clínica
/
Estudo de rastreamento
Idioma:
Inglês
Ano de publicação:
1997
Tipo de documento:
Artigo
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